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71636 Sigma-Aldrich

Sodium phosphate dibasic

BioUltra, for molecular biology, ≥99.5% (T)

Synonym: sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
Quality Level   200
grade   for molecular biology
product line   BioUltra
assay   ≥99.5% (T)
form   coarse powder
  fine crystals
  powder
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.001% total nitrogen (N)
loss   ≤0.1% loss on drying, 110 °C
pH   8.8-9.4 (25 °C, 0.5 M in H2O)
pKa (25 °C)   (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
solubility   H2O: 0.5 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤10 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.2 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤10 mg/kg
  Zn: ≤5 mg/kg
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.025
  λ: 280 nm Amax: ≤0.020
SMILES string   [Na+].[Na+].[H]OP([O-])([O-])=O
InChI   1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2
InChI key   BNIILDVGGAEEIG-UHFFFAOYSA-L

Description

Application

Sodium phosphate dibasic has been used:
• as a component in phosphate buffer
• in the preparation of MgCl2 hybridization buffer
• as a component in SSPE solution for the preparation of small RNA hybridisation buffer

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
WGK 1
RTECS 
WC4500000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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