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71736 Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H2O

Synonym: Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution

  • CAS Number 151-21-3

  • Linear Formula CH3(CH2)11OSO3Na

  • Molecular Weight 288.38

  •  Beilstein/REAXYS Number 3599286

  •  MDL number MFCD00036175

  •  eCl@ss 39093306

  •  PubChem Substance ID 57652355

  •  NACRES NA.26

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Properties

Related Categories Alkyl Sulfates, Alphabetical, Anionic Detergents, Antigen-Vaccine Preparation, BioUltra,
Quality Level   200
grade   for molecular biology
description   anionic
product line   BioUltra
mol wt   288.38 g/mol
concentration   10% in H2O
application(s)   HPLC: suitable
  protein quantification: suitable
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   5.0-6.5
density   1.01 g/mL at 20 °C
anion traces   chloride (Cl-): ≤50 mg/kg
  phosphate (PO43-): ≤1 mg/kg
cation traces   Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
λ   neat
UV absorption   λ: 260 nm Amax: 0.3
  λ: 280 nm Amax: 0.2
SMILES string   [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
InChI   1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
InChI key   DBMJMQXJHONAFJ-UHFFFAOYSA-M

Description

Application

Sodium dodecyl sulfate solution has been used:
• in the preparation of 2x extraction buffer
• as a component in elution buffer for immunoprecipitation
• in SDS–polyacrylamide gel electrophoresis (PAGE) analysis

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids. It is also considered as a permeability enhancer in oral formulations.

Other Notes

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Protocols

TRI Reagent® Protocol

TRI Reagent® solution (also sold as TRIzol) is a mixture of a mixture of guanidine thiocyanate and phenol in a monophase solution that is used for the isolation of DNA, RNA and protein from biologica...
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

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Peer-Reviewed Papers
15

References

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