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72485 Sigma-Aldrich

Nile Red

for microscopy

Synonym: Nile Blue A Oxazone

  • CAS Number 7385-67-3

  • Empirical Formula (Hill Notation) C20H18N2O2

  • Molecular Weight 318.37

  •  Beilstein/REAXYS Number 279110

  •  EC Number 230-966-0

  •  MDL number MFCD00011639

  •  PubChem Substance ID 57652419

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains, Luminescent Compounds/Detection, Other Fluorescent Labels,
grade   for microscopy
InChI Key   VOFUROIFQGPCGE-UHFFFAOYSA-N
mp   203-205 °C (lit.)
absorption   λmax 553 nm

Description

General description

Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets and proteins. Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells. It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.

Application

Nile Red has been used:
• as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)
• to label nanoparticles (NPs)
• for lipid analysis

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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