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72559 Sigma-Aldrich

Nitrilotriacetic acid

BioUltra, ≥99.0% (T)

Synonym: N,N-Bis(carboxymethyl)glycine, NTA, Tris(carboxymethyl)amine

  • CAS Number 139-13-9

  • Linear Formula N(CH2COOH)3

  • Molecular Weight 191.14

  •  Beilstein/REAXYS Number 1710776

  •  EC Number 205-355-7

  •  MDL number MFCD00004287

  •  PubChem Substance ID 57652424

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Properties

Related Categories Biochemicals and Reagents, Chelators More...
InChI Key   MGFYIUFZLHCRTH-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.0% (T)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.05%
loss   ≤0.1% loss on drying, 110 °C
mp   245 °C (dec.) (lit.)
solubility   0.1 M NaOH: 0.1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤100 mg/kg
cation traces   Al: ≤20 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤20 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤500 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.1 M in 1 M NaOH
UV absorption   λ: 260 nm Amax: 1.5
  λ: 280 nm Amax: 0.05

Description

Application

Nitrilotriacetic has been used in the preparation of nitrilotriacetic (NTA) solution for Ni-NTA slide generation.

An excellent chelating agent.

Biochem/physiol Actions

Nitrilotriacetic (NTA) has the ability to link with histidine side chain of proteins. It can be used in fluorescent labelling, hexahistidine (His6)-tagged proteins for purification and surface immobilization.

Other Notes

Chelating agent; As Pb2+ buffer, preparation and properties; In a mixture of Ca2+-buffering ligands; Encapsulation of radioactive metal ions in liposomes; Compilation and critical evaluation by IUPAC of published data on stability constants of NTA complexes in solution.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
AJ0175000
Flash Point(F) 
212 °F
Flash Point(C) 
100 °C

Documents

Certificate of Analysis

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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