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73144 Sigma-Aldrich

Resorufin

95%

Synonym: 7-Hydroxy-3H-phenoxazin-3-one, NSC 12097

  • CAS Number 635-78-9

  • Empirical Formula (Hill Notation) C12H7NO3

  • Molecular Weight 213.19

  •  Beilstein/REAXYS Number 174850

  •  EC Number 211-241-8

  •  MDL number MFCD00128991

  •  PubChem Substance ID 329764655

  •  NACRES NA.32

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Properties

Quality Level   100
assay   ≥95.0% (HPLC)
  95%
mp   >300 °C (lit.)
storage temp.   −20°C
SMILES string   O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2
InChI   1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H
InChI key   HSSLDCABUXLXKM-UHFFFAOYSA-N

Description

General description

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.

Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
SP7698000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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