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73677 Sigma-Aldrich

4-Nitrophenyl β-D-glucuronide

≥99.0% (TLC)

Synonym: 4-Nitrophenyl-β-D-glucopyranosiduronic acid, PNPG

  • CAS Number 10344-94-2

  • Empirical Formula (Hill Notation) C12H13NO9

  • Molecular Weight 315.23

  •  Beilstein/REAXYS Number 1438576

  •  EC Number 233-753-0

  •  MDL number MFCD00036210

  •  PubChem Substance ID 57652486

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Glucuronidase, beta-, Substrates by Enzyme More...
Quality Level   200
assay   ≥99.0% (TLC)
form   powder or crystals
optical activity   [α]20/D −102±3°, c = 1% in ethanol
impurities   ≤0.05% free 4-nitrophenol
  ≤7% water
solubility   H2O: 0.1 g/mL, clear, faintly yellow
suitability   suitable as substrate for β-glucuronidase test
storage temp.   −20°C
SMILES string   O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)[N+]([O-])=O
InChI   1S/C12H13NO9/c14-7-8(15)10(11(17)18)22-12(9(7)16)21-6-3-1-5(2-4-6)13(19)20/h1-4,7-10,12,14-16H,(H,17,18)/t7-,8-,9+,10-,12+/m0/s1
InChI key   QSUILVWOWLUOEU-GOVZDWNOSA-N

Description

Application

4-Nitrophenyl β-D-glucuronide has been used for determining the β-glucuronidase activity in urine samples, β-glucuronidase activity of bacteria in caecum content digesta and bovine liver. It has also been used to study the inhibitory effect of Phyllanthus amarus extracts on β-glucuronidase.

Substrates

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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