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73677 Sigma-Aldrich

4-Nitrophenyl β-D-glucuronide

≥99.0% (TLC)

Synonym: 4-Nitrophenyl-β-D-glucopyranosiduronic acid, PNPG

  • CAS Number 10344-94-2

  • Empirical Formula (Hill Notation) C12H13NO9

  • Molecular Weight 315.23

  •  Beilstein/REAXYS Number 1438576

  •  EC Number 233-753-0

  •  MDL number MFCD00036210

  •  PubChem Substance ID 57652486

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Properties

Related Categories Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Glucuronidase, beta-, Substrates by Enzyme More...
InChI Key   QSUILVWOWLUOEU-GOVZDWNOSA-N
assay   ≥99.0% (TLC)
optical activity   [α]20/D −102±3°, c = 1% in ethanol
impurities   ≤0.05% free 4-nitrophenol
  ≤7% water
solubility   H2O: 0.1 g/mL, clear, faintly yellow
suitability   suitable as substrate for β-glucuronidase test
storage temp.   −20°C

Description

Application

4-Nitrophenyl β-D-glucuronide has been used for determining the β-glucuronidase activity in urine samples, β-glucuronidase activity of bacteria in caecum content digesta and bovine liver. It has also been used to study the inhibitory effect of Phyllanthus amarus extracts on β-glucuronidase.

Substrates

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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