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73837 Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside

≥98.0% (HPLC)

Synonym: X-α-Glc, X-α-glucoside

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Properties

InChI Key   OPIFSICVWOWJMJ-HRNXZZBMSA-N
assay   ≥98.0% (HPLC)
optical activity   [α]/D 105.0±5.0°, c = 1 in DMF/H2O 1:1
storage temp.   −20°C

Description

General description

5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside, X-Gluc, is a indigogenic substrate for β- glucuronidase enzyme (GUS). It aids screening of transgenic plants based on GUS activity. GUS deesterifies X-Gluc into indoxyl derivative which upon oxidative polymerization results in the generation of blue indigotin dye.

Application

suitable as substrate for α-glucosidase; substrate used in the Druggan-Forsythe-Iversen medium (DFI agar) for the selective detection of Enterobacter sakazakii in infant milk

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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