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75633 Sigma-Aldrich

Osmium tetroxide solution

for electron microscopy, 2% in H2O

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Properties

Related Categories Chemical Synthesis, Osmium, Oxidizing Agents, Synthetic Reagents
Quality Level   100
grade   for electron microscopy
concentration   2% in H2O
density   1.0 g/mL at 20 °C
  1.0 g/mL at 20 °C
storage temp.   2-8°C
SMILES string   O=[Os](=O)(=O)=O
InChI   1S/4O.Os
InChI key   VUVGYHUDAICLFK-UHFFFAOYSA-N

Description

Application

Osmium tetroxide solution has been used as a fixative in electron microscopy.

Biochem/physiol Actions

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 3287 6.1 / PGII
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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