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77332 Sigma-Aldrich

Peroxidase from horseradish

lyophilized, powder, beige, ~150 U/mg

Synonym: Horseradish peroxidase

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Description

General description

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Application

Peroxidase from horseradish has been used:
• for isolation of N-Linked glycans
• as a constituent of D-amino acid oxidase (DAAO) buffer
• to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Other Notes

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Injectable Hydrogels for Cell Delivery and Tissue Regeneration

Prathamesh M. Kharkar,1 April M. Kloxin1,2* 1Materials Science and Engineering and 2Chemical and Biomolecular Engineering University of Delaware, Newark, DE 19716 USA *Email: akloxin@udel.edu
Keywords: Addition reactions, Adhesion, Angiogenesis, Biochemistry, Biomaterials, Building blocks, Cardiovascular, Catalysis, Cell proliferation, Click chemistry, Clinical, Cycloadditions, Degradations, Growth factors, Reversed-phase chromatography

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Peer-Reviewed Papers
15

References

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P8125 Peroxidase from horseradish, Type I, essentially salt-free, lyophilized powder, ≥50 units/mg solid (using pyrogallol)

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