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77332 Sigma-Aldrich

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

Synonym: Horseradish peroxidase



Related Categories 1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,
Quality Level   100
form   powder
quality   lyophilized
specific activity   ~150 U/mg
mol wt   Mr ~40000
solubility   50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)
shipped in   wet ice
storage temp.   2-8°C


General description

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.


Peroxidase from horseradish has been used:
• for isolation of N-Linked glycans
• as a constituent of D-amino acid oxidase (DAAO) buffer
• to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Other Notes

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Injectable Hydrogels for Cell Delivery and Tissue Regeneration

Prathamesh M. Kharkar,1 April M. Kloxin1,2* 1Materials Science and Engineering and 2Chemical and Biomolecular Engineering University of Delaware, Newark, DE 19716 USA *Email: akloxin@udel.edu
Keywords: Addition reactions, Adhesion, Angiogenesis, Biochemistry, Biomaterials, Building blocks, Cardiovascular, Catalysis, Cell proliferation, Click chemistry, Clinical, Cycloadditions, Degradations, Growth factors, Reversed-phase chromatography

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P8125 Peroxidase from horseradish, Type I, essentially salt-free, lyophilized powder, ≥50 units/mg solid (using pyrogallol)

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