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77617 Sigma-Aldrich

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 25:24:1

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Properties

Related Categories Acids and Bases, Alphabetical, BioUltra, Biochemicals and Reagents, Core Bioreagents,
Quality Level   200
grade   for molecular biology
product line   BioUltra
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
pH   7.7-8.3(H2O-phase, after extraction with H2O, 1:1)
density   1.28 g/mL at 20 °C
absorption   cut-off at 360 nm
storage temp.   2-8°C
SMILES string   ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
InChI   1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key   ZYWFEOZQIUMEGL-UHFFFAOYSA-N

Description

Application

Phenol − chloroform − isoamyl alcohol mixture has been used in nucleic acid extraction in bile duct tissue, Drosophila, chromatin and RNA immunoprecipitation in Caenorhabditis elegans and mitochondrial DNA.

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 25:24:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Other Notes

In the extraction of DNA for the generation of DNA minisatellite fingerprints

Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Central nervous system
RIDADR 
UN2810 - class 6.1 - PG 2 - EHS - Toxic, liquids, organic, n.o.s., HI: all
WGK Germany 
WGK 3
Flash Point(F) 
174.2 °F
Flash Point(C) 
79 °C

Documents

Certificate of Analysis (COA)

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pHast Pack
Protocols & Articles
Peer-Reviewed Papers
15

References

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