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77617 Sigma-Aldrich

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 25:24:1

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Properties

Related Categories Acids and Bases, Alphabetical, BioUltra, Biochemicals and Reagents, Core Bioreagents,
grade   for molecular biology
InChI Key   ZYWFEOZQIUMEGL-UHFFFAOYSA-N
product line   BioUltra
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
pH   7.7-8.3(H2O-phase, after extraction with H2O, 1:1)
density   1.28 g/mL at 20 °C
storage temp.   2-8°C

Description

Application

Phenol − chloroform − isoamyl alcohol mixture has been used in nucleic acid extraction in bile duct tissue, Drosophila, chromatin and RNA immunoprecipitation in Caenorhabditis elegans and mitochondrial DNA.

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 25:24:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Other Notes

In the extraction of DNA for the generation of DNA minisatellite fingerprints

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN2810 - class 6.1 - PG 2 - EHS - Toxic, liquids, organic, n.o.s., HI: all
WGK Germany 
3
Flash Point(F) 
174.2 °F
Flash Point(C) 
79 °C

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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