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  • 818M-9 - Cytokeratin 8 & 18 (B22.1 & B23.1) Mouse Monoclonal Antibody

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818M-9 Sigma-Aldrich

Cytokeratin 8 & 18 (B22.1 & B23.1) Mouse Monoclonal Antibody

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Properties

Related Categories Alphabetical Index, Anatomic Pathology, Antibodies, CT-CZ, Cell Marque Antibodies,
conjugate   unconjugated
clone   B22.1 & B23.1, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
packaging   vial of 0.1 mL concentrate (818M-94)
  vial of 0.5 mL concentrate (818M-95)
  bottle of 1.0 mL predilute (818M-97)
  vial of 1.0 mL concentrate (818M-96)
  bottle of 7.0 mL predilute (818M-98)
antibody form   diluted ascites fluid
isotype   IgG1κ
antibody product type   primary antibodies
Quality Level   GMP-IVD
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
mfr. no.   Cell Marque
concentration   1.12-2.08 μg/mL (predilute)
  112-208 μg/mL (concentrate)
control   prostate, salivary gland
visualization   cytoplasmic

Description

General description

Cytokeratins 8 & 18 (CK 8 & 18) can be found in most simple epithelia, e.g. thyroid, female breast, gastrointestinal tract, and respiratory tract. Adenocarcinomas and most non-keratinizing squamous carcinomas will stain with anti-CK 8 & 18, but keratinizing squamous carcinomas will not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so this will only stain Paget cells. The use of immunostaining facilitates the interpretation and has been shown to be more sensitive than mucin histochemistry.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Quality


IVD

IVD

IVD

RUO

Linkage

Cytokeratin 8 & 18 Positive Control Slides, Product No. 818S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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818S Cytokeratin 8 & 18 Positive Control Slides, suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) 

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