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83264 Sigma-Aldrich

HEPES buffer solution

1 M in H2O

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  MDL number MFCD00006158

  •  eCl@ss 32129211

  •  PubChem Substance ID 57652951

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z,
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
concentration   1 M in H2O
  238 g/L HEPES in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   5.5±0.5
density   1.07 g/mL at 20 °C
Featured Industry   Diagnostic Assay Manufacturing

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
• To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
• As a component of dissection buffer for culturing rat cortical neurons
• Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
• As a component of Danieu′s aquarium water used in agarose gel preparation

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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