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84097 Sigma

Sucrose

BioUltra, for molecular biology, ≥99.5% (HPLC)

Synonym: α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar

  • CAS Number 57-50-1

  • Empirical Formula (Hill Notation) C12H22O11

  • Molecular Weight 342.30

  •  Beilstein Registry Number 90825

  •  EC Number 200-334-9

  •  MDL number MFCD00006626

  •  PubChem Substance ID 57652987

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Biostabilization Agents, Carbohydrates,
grade   for molecular biology
InChI Key   CZMRCDWAGMRECN-UGDNZRGBSA-N
product line   BioUltra
assay   ≥99.5% (HPLC)
optical activity   [α]20/D +66.5±1°, c = 26% in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.01% (as SO4)
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   5.0-7.0 (25 °C, 1 M in H2O)
mp   185-187 °C(lit.)
solubility   H2O: 1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.11
  λ: 280 nm Amax: 0.08

Description

Other Notes

Isopycnic centrifugation in a discontinuous sucrose density gradient e.g. in the isolation of mRNA from membrane-bound polysomes

Application

Sucrose is a widely used nutitional disaccharide of glucose and fructose. Sucrose is used in molecular biology for the preparation of density gradients.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
WN6500000

High Quality GMP-Buffers

Protein Stabilizer
Protocols & Articles
Peer-Reviewed Papers
15

References

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