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86186 Sigma-Aldrich

Sulforhodamine B acid chloride

suitable for fluorescence, technical

Synonym: Lissamine rhodamine B sulfonylchloride

  • CAS Number 62796-29-6

  • Empirical Formula (Hill Notation) C27H29ClN2O6S2

  • Molecular Weight 577.11

  •  Beilstein/REAXYS Number 7734927

  •  EC Number 263-735-8

  •  MDL number MFCD00042001

  •  PubChem Substance ID 57653085

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Detection, Fluorescent Indicators and Probes, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains,
grade   technical
fluorescence   λex 560 nm; λem 580 nm in chloroform
  λex 560 nm; λem ~580 nm in chloroform
suitability   suitable for fluorescence
shipped in   wet ice
storage temp.   −20°C
SMILES string   CCN(CC)c1ccc2c(OC3=C\C(C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(Cl)(=O)=O)=[N+](/CC)CC)c1
InChI   1S/C27H29ClN2O6S2/c1-5-29(6-2)18-9-12-21-24(15-18)36-25-16-19(30(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(28,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3
InChI key   YERWMQJEYUIJBO-UHFFFAOYSA-N

Description

General description

Sulforhodamine B is a fluorescent dye.

Application

Sulforhodamine B acid chloride has been used to synthesize rhodamine-labeled polycation (PLL-ROD). It has also been used in fluorescent protein tracer preparation.

Fluorescent protein label forming stable conjugates

Biochem/physiol Actions

The sulforhodamine B (SRB) assay is useful in determining the density of cells.

Legal Information

Lissamine is a trademark of PerkinElmer Inc.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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