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86826 Sigma-Aldrich

5-Carboxyfluorescein

suitable for fluorescence, BioReagent, ≥95% (HPLC)

Synonym: 5-FAM

  • CAS Number 76823-03-5

  • Empirical Formula (Hill Notation) C21H12O7

  • Molecular Weight 376.32

  •  Beilstein/REAXYS Number 57037

  •  MDL number MFCD00036874

  •  PubChem Substance ID 57653195

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Properties

Related Categories Biochemicals and Reagents, Detection, Fluorescein and Derivatives, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains,
InChI Key   NJYVEMPWNAYQQN-UHFFFAOYSA-N
product line   BioReagent
assay   ≥95% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
  H2O: soluble
fluorescence   λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0
suitability   suitable for fluorescence

Description

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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