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90819 Sigma-Aldrich

Bis(2,2′-bipyridine)-(5-aminophenanthroline)ruthenium bis(hexafluorophosphate)

BioReagent, suitable for fluorescence

Synonym: Ru(bpy)2(phen-5-NH2)(PF6)2

  • CAS Number 84537-86-0

  • Empirical Formula (Hill Notation) C32H25N7Ru · 2 PF6

  • Molecular Weight 898.59

  •  MDL number MFCD20921517

  •  PubChem Substance ID 57653411

  •  NACRES NA.21

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Properties

Related Categories Biochemicals and Reagents, Catalysis and Inorganic Chemistry, Chemical Synthesis, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains,
Quality Level   100
product line   BioReagent
form   powder
application(s)   hematology: suitable
  histology: suitable
fluorescence   λex 458 nm; λem 612 nm in 0.1 M Tris pH 7.0
suitability   suitable for fluorescence
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   −20°C
SMILES string   [Ru++].F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.c1ccc(nc1)-c2ccccn2.c3ccc(nc3)-c4ccccn4.Nc5cc6cccnc6c7ncccc57
InChI   1S/C12H9N3.2C10H8N2.2F6P.Ru/c13-10-7-8-3-1-5-14-11(8)12-9(10)4-2-6-15-12;2*1-3-7-11-9(5-1)10-6-2-4-8-12-10;2*1-7(2,3,4,5)6;/h1-7H,13H2;2*1-8H;;;/q;;;2*-1;+2
InChI key   QQMMCXKCYXQNNA-UHFFFAOYSA-N

Description

Application

Used for photoinduced intramolecular electron transfer in peptide-bridged molecules.

Features and Benefits

Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds.
These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts.
In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Photoinduced intramolecular electron transfer in peptide-bridged molecules

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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904945 [Ru(dmbpy)3](PF6)2, ≥95%
904953 [Ru(dtbbpy)3](PF6)2, ≥95%

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