EMAIL THIS PAGE TO A FRIEND
91228 Sigma-Aldrich

Trichloroacetic acid

BioUltra, ≥99.5% (T)

Synonym: TCA

  • CAS Number 76-03-9

  • Linear Formula Cl3CCOOH

  • Molecular Weight 163.39

  •  Beilstein/REAXYS Number 970119

  •  EC Number 200-927-2

  •  MDL number MFCD00004177

  •  PubChem Substance ID 57653428

  •  NACRES NA.25

Purchase

Properties

Related Categories Acids and Bases, Alphabetical, Biochemicals and Reagents, Denaturation
Quality Level   200
vapor density   <1 (vs air)
vapor pressure   1 mmHg ( 51 °C)
product line   BioUltra
assay   ≥99.5% (T)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.05%
refractive index   n20/D 1.62 (lit.)
pH   <1.0 (25 °C, 0.5 M in H2O)
bp   196 °C (lit.)
mp   54-58 °C (lit.)
solubility   H2O: 0.5 M at 20 °C, clear, colorless
density   1.62 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤30 mg/kg
  nitrate (NO3-): ≤20 mg/kg
  phosphate (PO43-): ≤10 mg/kg
  sulfate (SO42-): ≤100 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: 1.60
  λ: 280 nm Amax: 0.15
SMILES string   OC(=O)C(Cl)(Cl)Cl
InChI   1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
InChI key   YNJBWRMUSHSURL-UHFFFAOYSA-N

Description

General description

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Application

Trichloroacetic acid (TCA) has been used:
• to determine its effect on avian embryos during an exposure window encompassing cardiac specification
• as a cell fixative
• to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
• for the precipitation of acidic ribosomal proteins (RPs)

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Other Notes

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Target organs 
Respiratory system
RIDADR 
UN 1839 8 / PGII
WGK Germany 
WGK 2
RTECS 
AJ7875000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Related Content

Carcinogen Selector - Digestive System - Liver

From our library of Related Content, Sigma-Aldrich presents Carcinogen Selector - Digestive System - Liver
Keywords: Cancer, Carcinogens, Respiratory

Carcinogen Selector - Endocrine System - Liver Endocrine

The Carcinogen Selector gives researchers the ability to select carcinogens by the mouse organs that they promote cancer in. The tool includes over 750 carcinogens that target 35 specific organs acro...
Keywords: Cancer, Carcinogens, Respiratory

Carcinogen Selector - Endocrine System

The Carcinogen Selector gives researchers the ability to select carcinogens by the mouse organs that they promote cancer in. The tool includes over 750 carcinogens that target 35 specific organs acro...
Keywords: Cancer, Carcinogens, Respiratory

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?