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92545 Sigma-Aldrich

Abberior® CAGE 552, maleimide

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

  • Empirical Formula (Hill Notation) C32H28N6O5

  • Molecular Weight 576.60

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Properties

Related Categories Abberior Dyes for Super-Resolution Microscopy, Biochemicals and Reagents, Luminescent Compounds/Detection More...
solubility   DMF: 0.25 mg/mL, clear
fluorescence   λex 552 nm; λem 580 nm±10 nm in PBS, pH 7.4
storage temp.   −20°C

Description

Analysis Note

degree of coupling: report results

General description

Abberior CAGE 552 is sold as a nonfluorescent and nearly colorless substance which, upon photolysis at 360-440 nm, readily transforms into the deeply red and highly fluorescent dye. It absorbs around 552 nm and emits around 575 nm. After uncaging it can be effectively used as a STED dye at ~660nm.
High brightness and a very good photostabilily are key features of the uncaged form of this dye. It is the smallest and lightest caged rhodamine dye available.

Abberior CAGE 552 dye is designed for single-molecule photoswitching (SMS) techniques such as PALM, STORM and GSDIM. In its uncaged variant it is also very suitable for STED microscopy. Abberior CAGE 552 can be applied in multilabel imaging based on the simultaneous use of 2 labels with a similar absorption and emission spectra. For that, Abberior CAGE 552 can be combined with the common TMR dye amd imaged only after uncaging. Another field of application of Abberior CAGE 552 are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.

Key Features
• High brightness and photostability
• Ideal for PALM, STORM, GSDIM
• Vers successfully tested with commercial microscopes

Absorption Maximum, λmax: 231, 308, 350 nm (non-activated, PBS, pH 7.4)
552 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 66,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 574 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength: 360 - 440 nm
Recommended STED Wavelength, λSTED: 650 − 670 nm
Fluorescence Quantum Yield, η: 0.37 (after photoactivation, PBS, pH 7.47)
Fluorescence Quantum Yield, η: 0.37 (pH 7)

Other Notes

Find more infomation here Abberior®

Application

• Abberior® CAGE 552 has been conjugated with secondary anti-guinea pig antibody for PALM (photo-activated light microscopy) imaging in MDA-MB-468 and COS-7 cells.
• It has been labelled with secondary antibody to perform dSTORM (direct stochastical optical reconstruction microscopy) for localization of deposited callose in destained Arabidopsis thaliana leaves.
• Abberior® CAGE 552 goat anti-mouse antibody has been used for FPALM (fluorescence photoactivation localization microscopy) imaging in NIH-3T3 fixed cells.
• It has also been used for PALM imaging of silica colloids.

Legal Information

abberior is a registered trademark of Abberior GmbH

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

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94822 Abberior® CAGE 552, NHS ester, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
53165 Anti-Mouse IgG−Abberior® CAGE 552 antibody produced in goat, for STED application
40544 Anti-Rabbit IgG−Abberior® CAGE 552 antibody produced in goat, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

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