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93014 Sigma-Aldrich

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium)

Green Alternative

powder, light brown, ≥10 U/g

Synonym: Manganese-dependent lignin peroxidase, MnP, Peroxidase, manganese

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Properties

Related Categories 1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Chemical Synthesis,
Quality Level   100
form   powder
greener alternative product characteristics   Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
color   light brown
greener alternative category   Enabling
shipped in   wet ice
storage temp.   −20°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell and cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium) is from the peroxidase family and is used to oxidize manganese. It may be used to study wound healing.

Biochem/physiol Actions

Manganese peroxidase catalyzes the oxidation of Mn2+ to Mn3+. Mn3+ is then in complex with oxalate, which is able to oxidize lignin. Manganese peroxidase from Phanerochaete chrysosporium binds 2 calcium ions per subunit. It binds 1 heme B (iron-protoporphyrin IX) group per subunit .

Unit Definition

One unit corresponds to the amount of enzyme, which oxidizes 1 μmole Mn2+ per minute to Mn3+ at pH 4.5 and 25 °C

Physical form

Supplied as a lyophilized powder containing ~50% sodium tartrate

only partially soluble in water or buffer

Other Notes

This product is supplied as a mixture of isozymes, mol. mass (40-65 kDa).

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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