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93337 Sigma-Aldrich

Trizma® acetate

BioUltra, ≥99.0% (NT)

Synonym: TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

  • CAS Number 6850-28-8

  • Linear Formula NH2C(CH2OH)3 · CH3COOH

  • Molecular Weight 181.19

  •  Beilstein/REAXYS Number 3702918

  •  EC Number 229-939-6

  •  MDL number MFCD00038951

  •  PubChem Substance ID 57653535

  •  NACRES NA.25

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Zwitterionic More...
Quality Level   200
product line   BioUltra
assay   ≥99.0% (NT)
form   crystalline powder
impurities   insoluble matter, passes filter test
ign. residue   ≤0.2%
loss   ≤0.2% loss on drying, 20 °C (HV)
pH   6.0-7.0 (25 °C, 0.5 M in H2O)
solubility   H2O: 0.5 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.5 M in H2O
UV absorption   λ: 260 nm Amax: 0.06
  λ: 280 nm Amax: 0.05
SMILES string   CC(O)=O.NC(CO)(CO)CO
InChI   1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)
InChI key   PIEPQKCYPFFYMG-UHFFFAOYSA-N

Description

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Other Notes

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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