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93422 Sigma-Aldrich

Triton X-114

Synonym: (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether

  • CAS Number 9036-19-5

  • Linear Formula (C2H4O)n C14H22O, n = 7 or 8

  •  Beilstein/REAXYS Number 2315025

  •  MDL number MFCD00128254

  •  PubChem Substance ID 57653556

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Properties

Related Categories Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents, Detergents A to M,
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
mol wt   average mol wt 537
impurities   ≤0.001% peroxides (as H2O2)
  ≤1% water
ign. residue   ≤0.2%
refractive index   n20/D 1.488 (lit.)
  n20/D 1.495
CMC   0.2 mM (20-25°C)
transition temp   pour point ~−9 °C
  cloud point 23 °C
density   1.06 g/mL at 20 °C
  1.058 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤500 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤500 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
HLB   12.4

Description

General description

Triton X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton X-114 has been used:
• In phase separation of integral membrane proteins
• In the purification of bacterial inclusion bodies
• To study the clouding phenomenon in promethazine hydrochloride, an amphiphilic phenothiazine drug
• To partition conditioned media into insoluble and soluble phases before Western blotting
• For solubilization of phosphatidylinositol 4-phosphate 5-kinase from bovine brain

Packaging

1 L in glass bottle

250 mL in glass bottle

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

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Single- & multi-channel pipettes from BRAND
Protocols & Articles
Peer-Reviewed Papers
15

References

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