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93443 Sigma-Aldrich

Triton X-100 solution

BioUltra, for molecular biology, ~10% in H2O

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether, Triton X-100

  • CAS Number 9002-93-1

  • Linear Formula t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10

  •  Beilstein/REAXYS Number 2315025

  •  MDL number MFCD00128254

  •  PubChem Substance ID 57653560

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Properties

Quality Level   200
grade   for molecular biology
description   non-ionic
product line   BioUltra
mol wt   micellar avg mol wt 80,000
  average mol wt 625
concentration   ~10% in H2O
aggregation number   100-155
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.349±0.002
pH   6.5-8.5 (25 °C)
CMC   0.2-0.9 mM (20-25°C)
  0.23 mM
transition temp   cloud point 65 °C
  pour point ~7 °C
density   1.01 g/mL at 20 °C
cation traces   Al: ≤1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤100 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Na: ≤100 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
absorption   cut-off at 310 nm
HLB   13.5
SMILES string   CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI   1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key   HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:
• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
• As a component of lysis buffer used for homogenization of cells
• As a component of blocking buffer in immunofluorescence staining
• For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
483.8 °F - closed cup
Flash Point(C) 
251 °C - closed cup

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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