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93443 Sigma-Aldrich

Triton X-100 solution

BioUltra, for molecular biology, ~10% in H2O

Synonym: 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution

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Properties

Related Categories Antigen-Vaccine Preparation, BioUltra, Biochemicals and Reagents, Cell Culture, DNA & RNA Purification,
grade   for molecular biology
description   non-ionic
InChI Key   HNLXNOZHXNSSPN-UHFFFAOYSA-N
product line   BioUltra
mol wt   ~647.0 g/mol
concentration   ~10% in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.349±0.002
pH   6.5-8.5 (25 °C)
CMC   0.23 mM
transition temp   cloud point 66  °C
density   1.01 g/mL at 20 °C
cation traces   Al: ≤1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤100 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Na: ≤100 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
absorption   absorption/310 nm cut-off
HLB   13.5

Description

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:
• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
• As a component of lysis buffer used for homogenization of cells
• As a component of blocking buffer in immunofluorescence staining
• For the preparation of cells in double fluorescence staining

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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