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93482 Sigma-Aldrich

Phenylmethanesulfonyl fluoride solution

~0.1 M in ethanol (T)

Synonym: Benzylsulfonyl fluoride, PMSF



Related Categories Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Type, Enzymes, Inhibitors, and Substrates, Organic Covalent Modifiers More...
Quality Level   200
biological source   microbial
form   liquid
concentration   ~0.1 M in ethanol (T)
density   0.797 g/mL at 20 °C
storage temp.   2-8°C
SMILES string   FS(=O)(=O)Cc1ccccc1
InChI   1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2



Phenylmethanesulfonyl fluoride solution has been used as a component in the cell lysate buffer in breast adenocarcinoma cell line, fibrosarcoma cell line and D5 hindbrain and spinal cord neural progenitors.

Biochem/physiol Actions

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.

Phenylmethanesulfonyl fluoride (PMSF) is a serine protease inhibitor. It binds covalently to active site residue of proteases and prevents proteolytic activity. PMSF is used in cell lysate buffers during protein purification to prevent target protein degradation. PMSF effectively inhibits the naloxone-precipitated withdrawal jumping.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN1170 - class 3 - PG 2 - Ethanol, solution
WGK Germany 
Flash Point(F) 
53.6 °F - closed cup
Flash Point(C) 
12 °C - closed cup


Certificate of Analysis (COA)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What canProduct 93482, Phenylmethanesulfonyl fluoride solution, be used for?
PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.
How can I further dilute Product 93482, Phenylmethanesulfonyl fluoride solution?
We would recommend that you use an anhydrous ethanol to further dilute this solution, since PMSF is very unstable in water.
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