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93773 Sigma-Aldrich

TWEEN® 20

viscosity 250-450 mPa.s (25 °C)

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
description   non-ionic
form   viscous liquid
mol wt   ~1228
application(s)   DNA extraction: suitable
  RNA extraction: suitable
  cell culture | mammalian: suitable
  protein purification: suitable
refractive index   n20/D 1.468 (lit.)
  n20/D 1.471
viscosity   250-450 mPa.s(25 °C)
HLB value   16.7±1.0
density   1.11 g/mL at 20 °C
  1.095 g/mL at 25 °C (lit.)

Description

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is widely used in several biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
• for lysing mammalian cells at a concentration of 0.005 to 0.5%
• For rehydrating tissue before in situ hybridization

Non-ionic detergent

Caution

may get turbid on storage, heat to clear

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TR7400000
Flash Point(F) 
>230 °F
Flash Point(C) 
>110 °C

Documents

Certificate of Analysis

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Single- & multi-channel pipettes from BRAND

Setting the Standard for Solid Formulation
Protocols & Articles
Peer-Reviewed Papers
15

References

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