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94506 Sigma-Aldrich

Tetramethylrhodamine-5-maleimide

suitable for fluorescence, ≥85% (HPLC)

  • CAS Number 174568-67-3

  • Empirical Formula (Hill Notation) C28H23N3O5

  • Molecular Weight 481.50

  •  MDL number MFCD01311091

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Fluorescence, Fluorescent Labeling of Sulfhydryls, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains,
assay   ≥85% (HPLC)
solubility   DMSO: soluble
  methanol: soluble
fluorescence   λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Application

Tetramethylrhodamine-5-maleimide has been used:
• to concentrate the eluate and in labeling to create IRE1LDS112C
• to derivatize green fluorescent protein (GFP) on Cys48 for experiments with the extrinsic fluorescent probe
• to incubate the single solvent exposed cys mutant to perform protein labeling

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Labeling reagent for protein thiols for cytofluorescence

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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