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94822 Sigma-Aldrich

Abberior® CAGE 552, NHS ester

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

  • Empirical Formula (Hill Notation) C30H25N5O6

  • Molecular Weight 551.55

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Properties

Related Categories Abberior Dyes for Super-Resolution Microscopy, Biochemicals and Reagents, Luminescent Compounds/Detection More...
assay   ≥80.0% (degree of coupling)
solubility   DMF: 0.25 mg/mL, clear
fluorescence   λex 552 nm; λem 584 nm±10 nm in PBS, pH 7
storage temp.   −20°C

Description

General description

Absorption Maximum, λmax: 231, 308, 350 nm (non-activated, PBS, pH 7.4)
552 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 66,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 574 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength: 360 - 440 nm
Recommended STED Wavelength, λSTED: 650 − 670 nm
Fluorescence Quantum Yield, η: 0.37 (after photoactivation, PBS, pH 7.47)

Other Notes

Find more infomation here Abberior®

Application

• Abberior® CAGE 552 has been conjugated with secondary anti-guinea pig antibody for PALM (photo-activated light microscopy) imaging in MDA-MB-468 and COS-7 cells.
• It has been labelled with secondary antibody to perform dSTORM (direct stochastical optical reconstruction microscopy) for localization of deposited callose in destained Arabidopsis thaliana leaves.
• Abberior® CAGE 552 goat anti-mouse antibody has been used for FPALM (fluorescence photoactivation localization microscopy) imaging in NIH-3T3 fixed cells.
• It has been used for PALM imaging of silica colloids.
• It has also been used for PALM imaging of functionally asymmetric GPCR (G protein-coupled receptor) oligomers.

Legal Information

abberior is a registered trademark of Abberior GmbH

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Description

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92545 Abberior® CAGE 552, maleimide, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
53165 Anti-Mouse IgG−Abberior® CAGE 552 antibody produced in goat, for STED application
40544 Anti-Rabbit IgG−Abberior® CAGE 552 antibody produced in goat, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

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