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96992 Sigma

Cell Counting Kit - 8

for quantitation of viable cell number in proliferation and cytotoxicity assays

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Properties

Related Categories Biochemicals and Reagents, Cell Culture, Cell Viability, Detection kits, Fluorescent and Colorimetric Assay Kits,
shelf life   limited shelf life, expiry date on the label
shipped in   ambient
storage temp.   2-8°C

Description

Application

Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Common Cell Culture Problems: Cell Clumping

Cells in suspension may attach to one another and form clumps for a variety of reasons. The most common cause of cell clumping is the presence of free DNA and cell debris in the culture medium, which...
Keywords: Adhesion, Apoptosis, Cell culture, Cell disruption, Culture media, Environmental, Flow cytometry

Common Cell Culture Problems: Cell Death

There is perhaps nothing more frustrating to a cell culturist than removing a dish from the incubator only to find that it’s full of dead cells. Getting to the bottom of the problem can be very chall...
Keywords: Antibiotics, Apoptosis, Autophagy, Buffers, Cell culture, Cell disruption, Cryopreservation, Culture media, Environmental, Enzyme activity, Filtration, Metabolism, Peptide synthesis, Reductions

Common Cell Culture Problems: Cell Line Cross-Contamination and Misidentification

A few years after scientists working at Johns Hopkins established the first human cell line using cervical epithelial cells (HeLa) from cancer patient Henrietta Lacks in the early 1950s, it was obser...
Keywords: Cancer, Cell culture, Cryopreservation, Forensic, Genetic, Phase transitions, Polymerase chain reaction

Common Cell Culture Problems: Contamination

Overview Types of Contamination Common Causes and Prevention of Contaminants    Microbial (bacteria, fungi, yeast)    Mycoplasma    Viral Contamination    Chemical Contamination General Tips and Tech...
Keywords: Antibiotics, Antifungals, Apoptosis, Buffers, Cell attachment, Cell culture, Culture media, Detergents, Eliminations, Environmental, Filtration, Fluorescent microscopy, Gene expression, Indicators, Metabolism, Microscopy, Polymerase chain reaction, Sterilizations

Common Cell Culture Problems: Poor Attachment of Adherent Cells

Optimal cell attachment in vitro requires the interaction of healthy cells with a wide range of cell-derived attachment molecules present in media, serum, and supplements. Cultureware surfaces are fr...
Keywords: Buffers, Cell attachment, Cell culture, Culture media, Decarboxylations, Environmental, Growth factors, Lipid peroxidation, Peroxidations, Precipitation, Reductions, Respiration

Common Cell Culture Problems: Precipitates

When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, a...
Keywords: Antibiotics, Cell culture, Culture media, Desiccation, Evaporation, Filtration, Microscopy, Nucleic acid denaturation, Precipitation, Purification

Protocols

Common Cell Culture Problems: Poor Cell Growth

Ensuring adequate cell growth is a critical part of collecting accurate data with cell cultures. Cells can be cultured in suspension, or as a monolayer that attaches to cultureware, such as a flask, ...
Keywords: Antibiotics, Apoptosis, Buffers, Cell attachment, Cell culture, Condensations, Cryopreservation, Culture media, Evaporation, Filtration, Microscopy, Phase transitions, Reductions

Peer-Reviewed Papers
15

References

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