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A2411 Sigma-Aldrich

Amphotericin B from Streptomyces sp.

BioReagent, suitable for cell culture, ~80% (HPLC)

Synonym: Fungizone

  • CAS Number 1397-89-3

  • Empirical Formula (Hill Notation) C47H73NO17

  • Molecular Weight 924.08

  •  Beilstein/REAXYS Number 78342

  •  EC Number 215-742-2

  •  MDL number MFCD00877763

  •  PubChem Substance ID 24890655

  •  NACRES NA.76

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics by Mechanism of Action, Antifungal, Biochemicals and Reagents,
Quality Level   300
biological source   Streptomyces sp.
InChI Key   APKFDSVGJQXUKY-INPOYWNPSA-N
product line   BioReagent
assay   ~80% (HPLC)
form   solid
potency   >750.00 μg per mg (Dry basis)
application(s)   cell culture | mammalian: suitable
color   yellow to yellow-orange
solubility   H2O: slightly soluble
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
storage temp.   2-8°C

Description

General description

Chemical structure: polyene

Packaging

1, 5 g in glass bottle

250 mg in glass bottle

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Caution

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Preparation Note

Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
BU2625000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibiotic Kill Curve

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers
15

References

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