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  • A3085 - 5-(N-Ethyl-N-isopropyl)amiloride

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A3085 Sigma-Aldrich

5-(N-Ethyl-N-isopropyl)amiloride

Synonym: EIPA

  • CAS Number 1154-25-2

  • Empirical Formula (Hill Notation) C11H18ClN7O

  • Molecular Weight 299.76

  •  MDL number MFCD00151710

  •  PubChem Substance ID 24278223

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Properties

Related Categories A to C, Adenosinetriphosphatase (Na/K), Amiloride and Derivatives, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules,
InChI Key   QDERNBXNXJCIQK-UHFFFAOYSA-N
mp   202-205 °C (lit.)
storage temp.   2-8°C
Gene Information   human ... SCNN1A(6337), SCNN1B(6338), SCNN1D(6339), SCNN1G(6340), SLC9A1(6548), SLC9A5(6553)
mouse ... Scnn1a(20276), Scnn1b(20277), Scnn1d(140501), Scnn1g(20278)
rat ... Scnn1a(25122), Scnn1b(24767), Scnn1g(24768), Slc9a1(24782), Slc9a2(24783), Slc9a3(24784), Slc9a4(24785)

Description

Application

5-(N-Ethyl-N-isopropyl)amiloride has been used:
• as a supplement in endothelial cell growth medium to incubate human lung microvascular endothelial cell
• in Pluronic F-127 stock solution for Drosophila larval live-imaging
• in choline chloride solution as an experimental perfusate, to study the effect of Na+/H+ inhibition on jejunal loop perfusion in vivo

Packaging

25, 100 mg in poly bottle

Biochem/physiol Actions

Selective blocker of Na+/H+ antiport

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How can I store a solution of5-(N-Ethyl-N-isopropyl)amiloride, Product A3085?
We do not have solution stability information for5-(N-Ethyl-N-isopropyl)amiloride.The product is sensitive to light, particularly in solution. In general, drug solutions are best prepared immediately before use.We recommend storing at -20°C in single use aliquots.
What can I use to make a solution of 5-(N-Ethyl-N-isopropyl)amiloride, Product A3085?
We test solubility of 5-(N-Ethyl-N-isopropyl)amiloride in methanol (10 mg/mL), yielding a clear yellow solution.It is also soluble in DMSO, and in dilute aqueous acid.It is insoluble in water.
What concentration ofProduct A3085,5-(N-Ethyl-N-isopropyl)amiloride, should I use in my experiment?
We have the following references for the use of5-(N-Ethyl-N-isopropyl)amiloride.A review article mentions the use of EIPA at 1 micromolar to probe Na+ channels in rat alveolar epithelial cells; see: Matalon, S., et al., Biophysical and molecular properties of amiloride-inhibitable Na+ channels in alveolar epithelial cells. Am. J. Physiol., 271, L1-L22 (1996).A study of the effects of EIPA on infarction in isolated rabbit hearts and cardiomyocytes has been reported; see: Sato, H., et al., The mechanism of protection from 5 (N-ethyl-N-isopropyl)amiloride differs from that of ischemic preconditioning in rabbit heart. Basic Res. Cardiol., 92, 339-350 (1997).
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Protocols & Articles

Articles

Discover Bioactive Small Molecules for Ion Channels Research

Ion transport across the relatively impermeable lipid bilayer of the cell membrane is accomplished via membrane proteins known as ion channels, pumps and transporters. Ion channels are gated pores an...
Keywords: Biological processes, Cell signaling, Diseases, Hormones, Ligands, Neurotransmitters

Peer-Reviewed Papers
15

References

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