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A3449 Supelco

Acrylamide/Bis-acrylamide, 30% solution

BioReagent, suitable for electrophoresis, 19:1

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Properties

Related Categories Acrylamides, Molecular Biology, Protein Electrophoresis, Proteomics, SDS-polyacrylamide gel electrophoresis (PAGE) More...
InChI Key   OCQZXMCGTAWGEQ-UHFFFAOYSA-N
sterility   0.2 μm filtered
product line   BioReagent
feed ratio   19:1
suitability   suitable for electrophoresis
storage temp.   2-8°C
  2-8°C

Description

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 5% stacking gel and 7% resolving gel for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Safety & Documentation

Safety Information

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Peer-Reviewed Papers
15

References

Related Products

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Description

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A9926 Acrylamide/bis-acrylamide, 40% solution, BioReagent, suitable for electrophoresis, 19:1
A2917 Acrylamide/Bis-acrylamide, BioReagent, for molecular biology, 19:1 (ratio)
08168 Timestrip Plus 8 °C

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