A4403 Sigma-Aldrich

L-Ascorbic acid

powder, suitable for cell culture, γ-irradiated

Synonym: L-Threoascorbic acid, Antiscorbutic factor, Vitamin C

  • CAS Number 50-81-7

  • Empirical Formula (Hill Notation) C6H8O6

  • Molecular Weight 176.12

  •  Beilstein/REAXYS Number 84272

  •  EC Number 200-066-2

  •  MDL number MFCD00064328

  •  PubChem Substance ID 24890857

  •  NACRES NA.75



Related Categories Amino Acids, Amino Acids & Vitamins, Amino Acids and Vitamins, Biochemicals and Reagents, Cell Culture,
Quality Level   200
biological source   synthetic (organic)
sterility   γ-irradiated
form   powder
mol wt   Mw 176.12 g/mol
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
  cell culture | plant: suitable
color   white to off-white
pH   1.0-2.5 (25 °C, 176 g/L in water)
mp   190-194 °C (dec.)
solubility   H2O: 10 mg/mL
SMILES string   OC([C@]([C@@H](O)CO)([H])O1)=C(O)C1=O
InChI   1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1
Gene Information   human ... SLC23A2(9962)


General description

L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.


For use in cell culture applications as an antioxidant and to regenerate tocopherol.
L-ascorbic acid has been used:
• in an osteogenic supplement for the growth of cells
• as a component in the differentiation media
• in WS-MSCs (Werner syndrome-mesenchymal stem cells) to study rescuing properties of ascorbic acid on aging defects

Biochem/physiol Actions

L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress. L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form). L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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