• USA Home
  • A7094 - L-Asparagine monohydrate

A7094 Sigma-Aldrich

L-Asparagine monohydrate

from non-animal source, BioReagent, suitable for cell culture, ≥98.0%

Synonym: (S)-2-Aminosuccinic acid 4-amide, (S)-(+)-2-Aminosuccinamic acid, L-Aspartic acid 4-amide

  • CAS Number 5794-13-8

  • Linear Formula NH2COCH2CH(NH2)COOH · H2O

  • Molecular Weight 150.13

  •  Beilstein/REAXYS Number 1723527

  •  EC Number 200-735-9

  •  MDL number MFCD00151038

  •  PubChem Substance ID 24891175



Related Categories Amino Acids, Amino Acids & Vitamins, Amino Acids and Vitamins, Cell Culture, Core Bioreagents,
biological source   non-animal source
product line   BioReagent
assay   ≥98.0%
form   powder
impurities   endotoxin, tested
mp   233-235 °C (lit.)
solubility   H2O: 20 mg/mL
suitability   suitable for cell culture



L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine. L-Asparagine, a proteinogenic amino acid, from non-animal source is used in biomanufacturing cell culture systems for the production of therapeutic recombinant proteins and monoclonal antibodies. It has also been used as a component of LHC basal media for the culture of human airway epithelial cells.


1 kg in poly bottle

25, 100 g in poly bottle

Biochem/physiol Actions

The amino acid L-asparagine is a structural analog of L-aspartic acid, where the side chain of the carboxylic acid moiety is amidated to give a terminal amine group. This renders L-asparagine neutral at physiological pH. The amide group of asparagine is derived from glutamine, in the reaction of aspartate and glutamine in the presence of ATP to yield asparagine and glutamate. In vivo, asparagine is hydrolyzed to aspartate and NH4+ by asparaginase. Asparagine is also an important amino acid in glycopeptide bonds, via N-glycosyl linkages to the sugar rings.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin


Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

A8381 L-Asparagine monohydrate, ≥99% (TLC)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?