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A8094 Sigma-Aldrich

L-Arginine

from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

Synonym: (S)-2-Amino-5-guanidinopentanoic acid

  • CAS Number 74-79-3

  • Linear Formula H2NC(=NH)NH(CH2)3CH(NH2)CO2H

  • Molecular Weight 174.20

  •  Beilstein/REAXYS Number 1725413

  •  EC Number 200-811-1

  •  MDL number MFCD00002635

  •  eCl@ss 32160406

  •  PubChem Substance ID 24891316

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Description

Application

L-Arginine has been used:
• as a Roswell park memorial institute medium (RPMI) media component in the isolation and culture of peripheral blood mononuclear cells (PBMCs)
• as a RPMI media component for tissue culture
• in DMEM medium for the identification and quantification of phosphorylation sites by stable isotope labeling by amino acids in cell culture (SILAC) and LCMS/MS

Packaging

1 kg in poly bottle

10 mg in glass bottle

25, 100 g in poly bottle

Biochem/physiol Actions

L-Arginine is a conditionally essential amino acid, that play a vital role in the production of nitric oxide. It acts as a precursor for the synthesis of polyamines, agmatine and urea. L-Arginine is usually essential in children and non-essential in adults.

Substrate of nitric oxide synthase, which is converted to citrulline and nitric oxide (NO). Induces insulin release by a nitric oxide-dependent mechanism.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
CF1934200

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND

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Protocols & Articles

Protocols

Protocol Guide: MTT Assay for Cell Viability and Proliferation

The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. This colorimetric assay is based on the reduction of a yellow tetrazoli...
Keywords: Antibiotics, Cancer, Cell proliferation, Enzyme-linked immunosorbent assay, Growth factors, Indicators, Metabolism, Reductions

Protocol Guide: XTT Assay for Cell Viability and Proliferation

The non-radioactive, colorimetric assay system using XTT (sodium 3´-[1- (phenylaminocarbonyl)- 3,4- tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) was first described by Scudiero,...
Keywords: Antibiotics, Cell proliferation, Enzyme-linked immunosorbent assay, Growth factors, Indicators, Metabolism, Reductions

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Peer-Reviewed Papers
15

References

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