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A8181 Sigma-Aldrich

Amido Black Staining Solution 2X

electrophoresis reagent

Synonym: Amido Black 10B, Naphthol Blue Black solution

  • CAS Number 1064-48-8

  • Empirical Formula (Hill Notation) C22H14N6Na2O9S2

  • Molecular Weight 616.49

  •  Beilstein/REAXYS Number 5374263

  •  MDL number MFCD00004017

  •  PubChem Substance ID 24891210

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Description

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Application

Amido Black Staining Solution 2X has been used in the staining:
• serum proteins present in the culture supernatant
• cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
• histones from chromatin samples

Reconstitution

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 2924 8(3) / PGIII
WGK Germany 
2
Flash Point(F) 
86 °F
Flash Point(C) 
30 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, we offer a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also pe...
Keywords: PAGE, Proteomics

Peer-Reviewed Papers
15

References

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