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A8582 Sigma-Aldrich

Antifoam SE-15

aqueous emulsion for bacterial and mammalian systems

  •  NACRES NA.85

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Properties

Related Categories Antifoams, Media Components, Microbial Media for Molecular Biology and Protein Expression, Molecular Biology
Quality Level   200
form   emulsion (aqueous)
concentration   10% (active silicon)
application(s)   cell culture | hybridoma: suitable
  microbiology: suitable
solubility   H2O: soluble (effective hot or cold.)
density   1.0 g/mL at 25 °C
storage temp.   room temp
suitability   (Mammalian (suspension))
  bacteria (fermentation)

Description

General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used in the preparation of ramified rolling circle amplification (RAM) amplification buffer. It has also been used to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.

Packaging

100, 500 g in glass bottle

Components

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

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Keywords: Antimicrobials, Culture media, Fermentation, Filtration, Molecular biology, Nucleic acid denaturation, Sample preparations, Separation

Peer-Reviewed Papers
15

References

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