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A8601 Sigma-Aldrich

G 418 disulfate salt

Biotechnology Performance Certified, suitable for cell culture, suitable for plant cell culture

Synonym: Antibiotic G418

  • CAS Number 108321-42-2

  • Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4

  • Molecular Weight 692.71

  •  MDL number MFCD00058314

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Properties

grade   Biotechnology Performance Certified
form   powder
potency   450 units/mg
mol wt   692.7
application(s)   cell culture | mammalian: suitable
  cell culture | plant: suitable
impurities   endotoxin, tested
color   white to off-white
solubility   water: soluble 50 mg/mL, clear to slightly hazy, colorless to faint yellow or tan
Mode of action   protein synthesis | interferes
storage temp.   2-8°C
SMILES string   OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
InChI   1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
InChI key   UHEPSJJJMTWUCP-NKCAIAFTSA-N

Description

General description

Chemical structure: aminoglycoside

Application

G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml.

Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those that are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.

Biochem/physiol Actions

Used for selection of G418 resistant transformed cells in molecular biology applications.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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A1720 G 418 disulfate salt, powder, BioReagent, suitable for cell culture

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