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A9655 Sigma-Aldrich

Auramine O

Dye content ≥80 %, certified by the Biological Stain Commission

Synonym: 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

  • CAS Number 2465-27-2

  • Empirical Formula (Hill Notation) C17H21N3 · HCl

  • Molecular Weight 303.83

  •  Colour Index Number 41000

  •  Beilstein/REAXYS Number 4030061

  •  EC Number 219-567-2

  •  MDL number MFCD00012484

  •  PubChem Substance ID 24891484

  •  NACRES NA.47

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Properties

Related Categories A, Biochemicals and Reagents, Cell Biology, Certified Stains & Dyes, Fluorescent Labels,
Quality Level   200
grade   certified by the Biological Stain Commission
form   powder
composition   Dye content, ≥80%
application(s)   hematology: suitable
  histology: suitable
mp   >250 °C (dec.) (lit.)
solubility   ethanol: 1 mg/mL, yellow to orange
λmax   370 nm in H2O
  432 nm in H2O
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C
InChI   1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
InChI key   KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

Packaging

25 g in glass bottle

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 2811 6.1 / PGIII
WGK Germany 
WGK 3
RTECS 
BY3675000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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