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ABK0001 Millipore

AvaBridge ELISA Kit

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
antibody form   IgG fraction of antiserum
species reactivity   human (Human IgG)
shipped in   wet ice
storage temp.   2-8°C

Description

Application

For research use only. Not for use in diagnostic procedures.

General description

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence of Bevacizumab. Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three dimensional structure of the protein and only a limited number of the antibodies respond. The result is a histogram which can be likened to a ′fingerprint′ for correctly folded Bevacizumab. For an Avastin Biosimilar, if the protein is correctly folded and glycosylated, the ′fingerprint′ will match that of Bevacizumab. If it is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the antibody made to that exposed sequence. In this way, changes in the ′fingerprint′ generated by the ELISA will point out differences between the Biosimilar and authentic Bevacizumab.

Safety & Documentation

Safety Information

RIDADR 
UN 3316 9

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protein Conformation Array Multiplexing

Monoclonal antibodies (mAbs) are the fastest growing class of human therapeutics within the field of biologics, having predicted worldwide sales of $125 billion by 2020 based on current approval rate...
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Protocols

AvaBridge ELISA Kit Protocol

Complete kit for the systematic 3-D conformational comparability analysis of Avastin biosimilar molecule to Bevacizumab (Avastin trade name). Read the entire protocol before performing the assay. Thi...
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Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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