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C0543 Sigma-Aldrich

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Type IV (Miller), lyophilized powder (from 0.2 μm-filtered solution), BioReagent, suitable for cell culture

Synonym: EHS-collagen

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Properties

Related Categories Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors, Biochemicals and Reagents,
biological source   mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
sterility   non-sterile
type   Type IV (Miller)
product line   BioReagent
form   lyophilized powder (from 0.2 μm-filtered solution)
packaging   glass bottle of 1 vial
surface coverage   6‑10 μg/cm2
solubility   0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)

Description

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

The substrate derived from engelbreth-holm-swarm murine sarcoma is laminin rich basement membrane. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. Type 4 collagen is a major component of basement membrane.

Other Notes

Native collagen

Packaging

1 vial in glass bottle

Preparation Note

Chloroform treated.
Pepsin is not used in the preparation.

Quantity

0.75 mg/vial

Application

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in adhesion assays.

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

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Cell Culture Guide
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Collagen Attachment Protocols, Solubility, and Stability

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Peer-Reviewed Papers
15

References

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