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C0662 Sigma-Aldrich

6-Carboxyfluorescein

~97% (HPLC)

Synonym: 6-FAM, FLUOS

  • CAS Number 3301-79-9

  • Empirical Formula (Hill Notation) C21H12O7

  • Molecular Weight 376.32

  •  Beilstein/REAXYS Number 54341

  •  MDL number MFCD00036873

  •  PubChem Substance ID 24892266

  •  NACRES NA.47

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Properties

Related Categories Biochemicals and Reagents, C, Cell Biology, Fluorescein and Derivatives, Fluorescent Labels,
Quality Level   200
assay   ~97% (HPLC)
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   >300 °C (lit.)
solubility   0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange
λmax   492 nm at 0.1 N in NaOH
fluorescence   λex 492 nm; λem 517 nm (pH 8.0)
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   2-8°C
SMILES string   OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
InChI   1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
InChI key   BZTDTCNHAFUJOG-UHFFFAOYSA-N

Description

Application

6-Carboxyfluorescein has been used to check the plasma membrane integrity in sperm cells.

Fluorescent dye.

Packaging

50 mg in poly bottle

Biochem/physiol Actions

6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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