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C0712 Sigma-Aldrich

CDP-Star® Chemiluminescent Substrate

0.25 mM in H2O

Synonym: Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate

  • CAS Number 160081-62-9

  • Empirical Formula (Hill Notation) C18H19Cl2O7Na2P

  • Molecular Weight 495.20

  •  MDL number MFCD02094611

  •  PubChem Substance ID 329774818

  •  NACRES NA.83

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Properties

Related Categories Alkaline Phosphatase, Alkaline Phosphatase Substrates for Western Blotting, Alphabetical Listing, Biochemicals and Reagents, Chemiluminescent,
Quality Level   200
assay   ≥75% (HPLC)
form   liquid
usage   sufficient for 20 blots detection of alkaline phosphatase-labeled molecules (10 × 10 cm)
concentration   0.25 mM in H2O
storage temp.   2-8°C
SMILES string   Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3
InChI   1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;
InChI key   UKWLRLAKGMZXJC-VCCHNUGISA-L

Description

Application

CDP-Star® is a sensitive, chemiluminescent substrate for alkaline phosphatase that allows for the rapid, reproducible detection of alkaline phosphatase-labeled molecules in Northern, Southern, and Western blotting applications. Detection of alkaline phosphatase-labeled molecules with CDP-Star is extremely sensitive as a result of low background luminescence coupled with high intensity and prolonged light output from the enzyme catalysis. Following exposure to alkaline phosphatase-labeled molecules, maximum light emission occurs at approximately 60 minutes and continues for up to 24 hours, allowing for multiple film exposures and/or the sensitive detection of targets present in small amounts. For convenience, CDP-Star is supplied as a 0.25 mM, ready-to-use aqueous solution (i.e. no dilution is necessary). CDP-Star functions on both neutral and positively-charged nylon, giving the reagent added application versatility.

Features and Benefits

CDP-Star® is supplied as a 0.25 mM ready-to-use aqueous solution for use in a variety of membrane-based applications. CDP-Star functions on both neutrally-charged and positively-charged nylon, giving the reagent added application versatility.

Caution

Store at 2-8 °C protected from light.

Legal Information

CDP-Star® is a trademark of Tropix, Inc. Bedford, MA, USA and covered under U.S. Patent 5,326,882.

CDP-Star is a registered trademark of Tropix, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

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