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C0775 Sigma-Aldrich

Crystal Violet

certified by the Biological Stain Commission

Synonym: Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

  • CAS Number 548-62-9

  • Linear Formula C25H30N3Cl

  • Molecular Weight 407.98

  •  Colour Index Number 42555

  •  Beilstein Registry Number 3580948

  •  EC Number 208-953-6

  •  MDL number MFCD00011750

  •  PubChem Substance ID 57653980

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal, Biochemicals and Reagents,
grade   certified by the Biological Stain Commission
InChI Key   ZXJXZNDDNMQXFV-UHFFFAOYSA-M
form   powder
composition   Dye content, ≥90%
mp   205 °C (dec.) (lit.)
solubility   H2O: 1 mg/mL
antibiotic activity spectrum   fungi

Description

Packaging

25, 100 g in glass bottle

Application

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Suitability

Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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