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C1809 Sigma

Collagen from kangaroo tail

Bornstein and Traub Type I (Sigma Type I), powder, cell culture tested

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Properties

form   powder
surface coverage   6‑10 μg/cm2
solubility   aqueous acid: soluble (1 - 2μl acetic acid per ml H2O)
suitability   cell culture tested
storage temp.   2-8°C

Description

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Preparation Note

Prepared by a modification of the pepsin extraction method of Niyibizi, et al., J. Biol. Chem., 259, 14170 (1984).

Physical form

Lyophilized from 10 mM glacial acetic acid

Application

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content. Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues. It is often used in cell culture as an attachment substratum. Myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have been cultured successfully on films or gels of Type I collagen. Type I collagen differs from other collagens by their low lysine hydroxylation and low carbohydrate composition.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

3d Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
Keywords: Alkylations, Apoptosis, Biomaterials, Cancer, Cell culture, Cell proliferation, Clinical, Cytotoxins, Drug discovery, Gene expression, Metabolism, Pharmaceutical

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Collagen Attachment Protocols, Solubility, and Stability

A1) We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of...
George Sitterley
BioFiles 2008, 3.8, 5.
Keywords: Centrifugation, Dialysis, Filtration, Purification, Sterilizations

Collagen Usage Recommendations

Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a num...
George Sitterley
BioFiles 2008, 3.8, 4.
Keywords: Cell culture, Morphogenesis

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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