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C1988 Sigma-Aldrich

Cycloheximide

Biotechnology Performance Certified

Synonym: 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide, Actidione, Naramycin A

  • CAS Number 66-81-9

  • Empirical Formula (Hill Notation) C15H23NO4

  • Molecular Weight 281.35

  •  Beilstein/REAXYS Number 88868

  •  EC Number 200-636-0

  •  MDL number MFCD00082346

  •  PubChem Substance ID 24892457

  •  NACRES NA.85

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics by Mechanism of Action, Antifungal, Biochemicals and Reagents,
grade   Biotechnology Performance Certified
InChI Key   YPHMISFOHDHNIV-FSZOTQKASA-N
form   powder
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
solubility   ethanol: 50 mg/mL
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   fungi
  yeast
storage temp.   2-8°C
Gene Information   human ... FKBP1A(2280), PIN1(5300)

Description

Application

Cycloheximide is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors. It has been shown to selectively clear macrophages in atherosclerotic plaques and activate cumulus-free equine oocytes.

Packaging

1, 5 g in glass bottle

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Cycloheximide (CHX) is an antibiotic produced by S. griseus that inhibits protein biosynthesis in eukaryotes. It inactivate transferase II enzyme that is involved in peptide chain elongation. More recent studies revealed that CHX binds the 60S ribosome and specifically prevents the translocation step in elongation.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN2811 - class 6.1 - PG 2 - EHS - Toxic solids, organic, n.o.s., HI: all
WGK Germany 
3
RTECS 
MA4375000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Peer-Reviewed Papers
15

References

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