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C4243 Sigma-Aldrich

Collagen solution from bovine skin

BioReagent, suitable for cell culture, sterile-filtered

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Properties

Related Categories Attachment Factors, Attachments Factors, Cell Culture, Core Bioreagents, Life Science Reagents for Cell Culture,
biological source   bovine skin
sterility   sterile-filtered
product line   BioReagent
assay   ≥99.9% (SDS-PAGE)
form   liquid
packaging   pkg of 100 mL
  pkg of 20 mL
surface coverage   6‑10 μg/cm2
suitability   gelation test tested
  suitable for cell culture
foreign activity   endotoxin ≤1.0 μmol/mg protein
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... COL1A1(282187)

Description

Components

Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. As a heterodimer composed of two a1 chains and one a2 chains, it spontaneously forms a triple helix scaffold at neutral pH and 37°C.

Application

This highly purified solution is suitable for 3-D matrix formation in cell culture. 3-D collagen gels imitate the in vivo cell physiology better than traditional 2D systems and has been proven successful for several cell types including cardian and corneal fibroblasts, depatic stellate cells, and neuroblastoma cells. Such 3-D gels are also useful in studies of mechanotransduction, cell signaling involving the transformation of mechanical signals into biochemical signals

Collagen solution from bovine skin has been used in the preparation of collagen gels.

Biochem/physiol Actions

In 3D environments, cell extensions can use integrins on cell surfaces to activate specific signaling pathways and integran-independent mechanical interactions resulting from the entanglement of matrix fibrils is possible.

Collagen, type I (COL1A1) participates in fibrosis. COL1A1 is an important component of the connective tissue matrix. It plays a vital role in the growth and maintenance of organ and tissue integrity. This protein also participates in the process of tissue repair.

Caution

This product ships on wet ice and with recommended storage at 2-8°C, the product will last for 2 years.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Preparation Note

This product is prepared from type I bovine collagen purified and extracted from tendon and skin and contains a high monomer content. The raw collagen used to prepare this product has been isolated from a closed herd and purified with a GMP manufacturing process that includes inactivation of any possible prion or viral contamination. As supplied, it is a 3 mg/mL aqueous solution in 0.01 M HCl with a pH of 2.0. Collagen denatures when exposed to high temperatures or irradiation. Prior to a pH adjustment, store stock or diluted solutions refrigerated. Follwing a pH adjustment to 7, solutions should not exceed 40°C and should not be frozen.

General description

Collagen solution is derived by dissolving collagen molecules in an aqueous solution. Collagen type Iα1 (COL1A1) is encoded by the gene that is located on human chromosome 17q21.33. It is the most abundant extracellular matrix (ECM) protein in humans. Type 1 collagen is the major structural protein of bone, tendon, skin and cornea. The encoded protein is a heterotrimer consisting of two α1-chains and one α2-chain.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers
15

References

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