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C6158 Sigma-Aldrich

Crystal Violet

ACS reagent, ≥90.0% anhydrous basis

Synonym: Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

  • CAS Number 548-62-9

  • Linear Formula C25H30N3Cl

  • Molecular Weight 407.98

  •  Colour Index Number 42555

  •  Beilstein/REAXYS Number 3580948

  •  EC Number 208-953-6

  •  MDL number MFCD00011750

  •  PubChem Substance ID 57654029

  •  NACRES NA.47

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Properties

Related Categories C, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   200
grade   ACS reagent
InChI Key   ZXJXZNDDNMQXFV-UHFFFAOYSA-M
assay   ≥90.0% anhydrous basis
form   powder
loss   ≤7.5% loss on drying
mp   205 °C (dec.) (lit.)
solubility   water: soluble 50 g/L at 27 °C
absorption   passes test
suitability   passes test for sensitivity as indicator
Mode of action   cell membrane | interferes
  enzyme | inhibits
antibiotic activity spectrum   fungi

Description

Application

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Packaging

50, 100 g in glass bottle

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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