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C9377 Sigma-Aldrich

Chenodeoxycholic acid


Synonym: 3α,7α-Dihydroxy-5β-cholanic acid, 5β-Cholanic acid-3α,7α-diol, Chenodiol

  • CAS Number 474-25-9

  • Empirical Formula (Hill Notation) C24H40O4

  • Molecular Weight 392.57

  •  Beilstein/REAXYS Number 3219887

  •  EC Number 207-481-8

  •  MDL number MFCD00064142

  •  PubChem Substance ID 24893153

  •  NACRES NA.25



Related Categories Anionic Detergents, Bile Salts, Bile acids and Derivatives, Biochemicals and Reagents, Cell Culture,
Quality Level   200
biological source   synthetic (organic)
description   anionic
assay   ≥96%
form   powder
mol wt   392.57 g/mol
application(s)   protein quantification: suitable
CMC   3
mp   165-167 °C (lit.)
functional group   carboxylic acid
shipped in   ambient
storage temp.   room temp
SMILES string   C[C@H](CCC(O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
InChI   1S/C24H40O4/c1-14(4-7-21(27)28)17-5-6-18-22-19(9-11-24(17,18)3)23(2)10-8-16(25)12-15(23)13-20(22)26/h14-20,22,25-26H,4-13H2,1-3H3,(H,27,28)/t14-,15+,16-,17-,18+,19+,20-,22+,23+,24-/m1/s1
Gene Information   human ... CYP1A2(1544), NR1H4(9971)


General description

Chenodeoxycholic acid is a bile acid synthesized in the liver from cholesterol.


Chenodeoxycholic acid has been used in a study to assess its effects as a long-term replacement therapy for cerebrotendinous xanthomatosis (CTX). It has also been used in a study to investigate its effects on the small-intestinal absorption of bile acids in patients with ileostomies.


100 mg in glass bottle

5, 25 g in glass bottle

Biochem/physiol Actions

Bile Acid

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. C9377.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


BACSMLS Online Plate Map

BACSMLS compounds are conveniently provided at 5µg per well, enough for multiple injections. These are high purity (>95%) compounds supplied in an economical, ready to use format. Compounds are arran...
Keywords: Metabolites

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Fast and Robust HPLC Separation of Bile Acids and Conjugates with Ascentis Express C18

Bile acids are increasingly recognized as playing an important signaling role in the control of immune response and energy metabolism. Recently, there has been interest in measuring bile acid concent...
Clare Glicksman, Michael Wright, Dave Bell, and Anders Fridström
Reporter US, Volume 33.1
Keywords: Bacterial conjugations, Clinical, High performance liquid chromatography, Hormones, Mass spectrometry, Metabolism, Separation, Thin layer chromatography

HPLC Analysis of Bile Acids on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Bile Acids on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography

LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

Peer-Reviewed Papers


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Add to Cart

614122 Chenodeoxycholic acid-2,2,4,4-d4, 98 atom % D, 98% (CP)

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