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C9426 Sigma-Aldrich

CHAPS hydrate

BioReagent, suitable for electrophoresis, ≥98% (HPLC)

Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

  • CAS Number 331717-45-4

  • Empirical Formula (Hill Notation) C32H58N2O7S · xH2O

  • Molecular Weight 614.88 (anhydrous basis)

  •  PubChem Substance ID 24892997

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Buffers, Cell Culture, Core Bioreagents,
description   zwitterionic
InChI Key   SJCUTFKCLFLIFE-JWTJKVBLSA-N
product line   BioReagent
assay   ≥98% (HPLC)
mol wt   614.88 g/mol (anhydrous basis)
application(s)   protein quantification: suitable
mp   157 °C (dec.) (lit.)
suitability   suitable for electrophoresis

Description

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

CHAPS has been used:
• For membrane permeabilization of cells
• As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue
• As a component in denaturing buffer in enzyme characterization
• As a component of 2D- sample buffer in 2D electrophoresis
• For protein solubilization in isoelectric focusing

Packaging

1, 5 g in glass bottle

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles
Peer-Reviewed Papers
15

References

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