• USA Home
  • CRISPR15H - Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)

EMAIL THIS PAGE TO A FRIEND
CRISPR15H Sigma-Aldrich

Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)

Purchase

Properties

Related Categories CRISPR Controls (DNA and Virus), CRISPR-Cas9, Functional Genomics and RNAi, Molecular Biology
packaging   vial of 8 x 25 μL
concentration   (5x108 TU/ml (via p24 titering assay))
application(s)   CRISPR: suitable
shipped in   dry ice
storage temp.   −70°C

Description

Application

Functional Genomics/Screening/Target Validation

General description

The Lenti CRISPR Non-Target gRNA Control Transduction Particles (LV02 vector) include a gRNA sequence that does not target known human, mouse and rat genes. It is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-target) and Ef1a-driven Puromycin resistance cassette. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Legal Information

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

Other Notes

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9

Documents

Certificate of Analysis

Protocols & Articles

Related Content

CRISPR

In recent years CRISPR has revolutionized gene editing capabilities, leading to sophisticated ways to create success with any experiment. As the first company to offer custom biomolecules globally fo...
Keywords: Genetic, Transduction, Transfection

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?