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D0550 Sigma-Aldrich

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym: 3,5-Dinitro-2-hydroxybenzoic acid, DNS

  • CAS Number 609-99-4

  • Linear Formula (O2N)2C6H2-2-(OH)CO2H

  • Molecular Weight 228.12

  •  Beilstein/REAXYS Number 2220661

  •  EC Number 210-204-3

  •  MDL number MFCD00007104

  •  PubChem Substance ID 24893243

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Properties

Related Categories Cell Biology, D, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   200
assay   ≥98% (HPLC and titration)
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   168-172 °C (lit.)
solubility   H2O: 50 mg/mL
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
InChI   1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
InChI key   LWFUFLREGJMOIZ-UHFFFAOYSA-N

Description

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Packaging

10, 25, 100, 500 g in glass bottle

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
VO2965000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Enzymatic Assay of Alpha-Amylase

The spectrophotometric stop reaction determination (A540, Light path = 1 cm) is based on the following reaction:

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

3.2. Unit Definition – One unit will liberate 1.0 mg of maltose from starch in 3 minutes at pH 4.8 at 20°C.
Keywords: Enzyme activity, Enzymology

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Peer-Reviewed Papers
15

References

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